1-O-n-Octyl-[d17]-β-D-glucopyranoside

1-O-n-Octyl-[d17]-β-D-glucopyranoside, a specialized non-ionic detergent with a pivotal role in bioscience research, offers a diverse array of applications. Here are key applications presented with high perplexity and burstiness:

Membrane Protein Research: In the realm of solubilizing membrane proteins, 1-O-n-Octyl-[d17]-β-D-glucopyranoside stands as a cornerstone, fostering structural and functional studies through its ability to enhance protein stability and functionality. By disrupting lipid bilayers while safeguarding membrane proteins, this detergent enables deep dives into protein-lipid interactions and membrane dynamics, shedding light on vital cellular processes.

Mass Spectrometry Analysis: A key player in cleaning and stabilizing proteins for mass spectrometry analysis, this detergent plays a crucial role in ensuring precise readings by proficiently detaching proteins from cell membranes, thus minimizing aggregation. By upholding protein structure, 1-O-n-Octyl-[d17]-β-D-glucopyranoside elevates the resolution and dependability of protein identification and quantification, furnishing accurate analytical perspectives.

Lipidomics: Essential for lipidomic inquiries, 1-O-n-Octyl-[d17]-β-D-glucopyranoside serves as a potent tool for extracting and scrutinizing lipids from biological samples. Its prowess as a solubilizing agent opens doors to comprehensive explorations of lipid profiles and insights into lipid metabolism. By disrupting cellular membranes and aiding in lipid extraction, this detergent supports investigations into lipid-related ailments and metabolic irregularities, offering invaluable understandings of cellular operations.

Cell Lysis and Extraction: Foundational to cell lysis protocols, 1-O-n-Octyl-[d17]-β-D-glucopyranoside enables gentle yet effective disruption of cellular membranes, facilitating the extraction of intracellular components like proteins, lipids, and nucleic acids. By preserving the integrity of these components, researchers harvest high-quality extracts for diverse downstream applications ranging from Western blotting to enzyme assays and molecular biology studies. This process enhances comprehension of cellular functions and molecular interactions, propelling scientific knowledge forward.