2-Aminoisobutyric acid-[15N]

2-Aminoisobutyric acid-[15N], a stable isotope-labeled compound, is renowned for its distinctive labeling, making it a staple in scientific research. Here are key applications of 2-Aminoisobutyric acid-[15N] presented with high perplexity and burstiness:

Metabolic Tracing: Within metabolic investigations, 2-Aminoisobutyric acid-[15N] shines as a pivotal tool for tracing nitrogen turnover and elucidating metabolic pathways in biological systems. The integration of the ^15N label permits researchers to meticulously follow its metabolic journey within organisms, offering profound insights into amino acid metabolism and nitrogen equilibrium across diverse cell types.

Protein Synthesis Studies: At the forefront of protein synthesis research, 2-Aminoisobutyric acid-[15N] plays a crucial role in unlocking the intricacies of protein dynamics and turnover. By incorporating this isotope-labeled amino acid into proteins, scientists can meticulously gauge shifts in protein synthesis rates under varying conditions, shedding light on protein metabolism and degradation mechanisms within cells with unprecedented clarity.

NMR Spectroscopy: The presence of the ^15N label in 2-Aminoisobutyric acid-[15N] elevates its utility in nuclear magnetic resonance (NMR) spectroscopy studies, offering a rich tapestry of molecular insights. This compound empowers researchers to delve into molecular structures and interactions through the provision of supplementary signals in the NMR spectrum. Scientists leverage this compound to explore protein-ligand interactions and unravel the intricate folds of protein structure, pushing the boundaries of structural elucidation.

Stable Isotope Labeling in Mass Spectrometry: In the realm of mass spectrometry, 2-Aminoisobutyric acid-[15N] serves as an indispensable internal standard for quantifying amino acids in complex biological samples. The unique isotopic pattern enhances the accuracy of analyte concentration measurements, elevating data reliability and precision in metabolomics and proteomics studies. This approach stands as a cornerstone for achieving absolute quantification of biomolecules, unlocking new avenues for understanding the molecular signatures within biological systems.