Boc-Gly-OH-[13C2,15N]

Boc-Gly-OH-[13C2,15N], an amino acid derivative enriched with stable isotopes, finds widespread utility across scientific research disciplines. Here are key applications of Boc-Gly-OH-[13C2,15N] presented with a high degree of perplexity and burstiness:

Protein NMR Studies: A cornerstone in structural biology, Boc-Gly-OH-[13C2,15N] emerges as a linchpin in nuclear magnetic resonance (NMR) spectroscopy, enabling the intricate exploration of protein architectures and dynamics. By integrating this isotopically labeled glycine into protein matrices, scientists unlock a trove of information on conformations and molecular interactions. This strategic maneuver elevates the acuity and sensitivity of NMR analyses, propelling the unraveling of convoluted biomolecular structures.

Metabolic Flux Analysis: Embedded within the realm of metabolic investigations, Boc-Gly-OH-[13C2,15N] assumes the role of a versatile tracer to decipher the labyrinthine pathways of cellular metabolism. Once introduced into biological systems, the labeled carbon and nitrogen atoms embark on a journey through metabolic cascades, shedding light on the flux and metamorphosis of essential nutrients. This knowledge forms the bedrock for understanding cellular energetics and pinpointing potential targets for metabolic engineering interventions.

Mass Spectrometry: A steadfast ally in the realm of mass spectrometry, Boc-Gly-OH-[13C2,15N] emerges as a pivotal tool for precise quantitation and identification of peptides and proteins. The incorporation of stable isotopes bolsters the discernment of labeled peptides amidst the intricate tapestry of biological mixtures. This bolstering of sensitivity enhances proteomics investigations by furnishing accurate quantitative insights into protein abundance and modifications, paving the path for transformative discoveries.

Analytical Chemistry: In the realm of analytical chemistry, Boc-Gly-OH-[13C2,15N] stands as a beacon of precision, serving as an indispensable reference standard for calibrating instruments and validating methodologies. Adorned with isotopic signatures, this derivative imparts a distinctive aura that sets it apart from natural isotopologues. This distinctiveness ensures heightened accuracy in analytical measurements, fortifying method validation endeavors and underpinning the advancement of analytical protocols.