D-fructose-[1-13C,1,1'-d2]

D-fructose-[1-13C,1,1'-d2], a stable isotope-labeled compound, finds diverse applications across bioscience. Here are the key applications of D-fructose-[1-13C,1,1'-d2]:

Metabolic Flux Analysis: In the realm of metabolic flux analysis, D-fructose-[1-13C,1,1'-d2] plays a crucial role in tracing carbon flow through intricate metabolic pathways. By integrating this labeled fructose into metabolic pathways, researchers can quantitatively assess fluxes and unravel the impact of metabolic alterations on cellular function. This technique is essential for optimizing metabolic engineering strategies and unraveling the dynamics of disease metabolism.

NMR Spectroscopy: Within the domain of nuclear magnetic resonance (NMR) spectroscopy, D-fructose-[1-13C,1,1'-d2] acts as a powerful tracer for studying the structure and dynamics of fructose metabolism within cells. The presence of 13C and deuterium labels introduces distinct signals that aid in mapping metabolic pathways and deciphering enzyme kinetics. These insights shed light on the intricate biochemical mechanisms and interactions that govern cellular processes.

Stable Isotope-Resolved Metabolomics: D-fructose-[1-13C,1,1'-d2] is a valuable asset in stable isotope-resolved metabolomics (SIRM), facilitating investigations into the metabolic destiny of fructose within biological systems. By tracking the incorporation of labeled carbons into various metabolites, researchers gain detailed insights into metabolic cycles and pathway activities. SIRM serves as a powerful tool for dissecting the impacts of diet, disease, and genetic modifications on metabolism, offering profound understanding into cellular metabolism.

Diabetes Research: Within the realm of diabetes research, D-fructose-[1-13C,1,1'-d2] emerges as a pivotal player in studying fructose metabolism and its implications on insulin resistance and glucose homeostasis. By integrating this labeled fructose into experimental setups, scientists deepen their comprehension of how fructose contributes to metabolic disorders. This knowledge serves as a cornerstone for crafting dietary guidelines and formulating therapeutic interventions aimed at managing diabetes effectively.