D-ribitol-[1-13C]

D-ribitol-[1-13C], an isotopically labeled sugar alcohol, finds diverse scientific applications. Here are the key applications of D-ribitol-[1-13C]:

Metabolic Flux Analysis: Deployed in metabolic flux analysis, D-ribitol-[1-13C] serves to trace the flow of carbon within metabolic pathways. By introducing this labeled compound into cell cultures, researchers can meticulously track its integration into various metabolic products using advanced techniques like mass spectrometry or nuclear magnetic resonance (NMR). This approach aids in unraveling the intricacies of cellular metabolism and pinpointing metabolic bottlenecks that govern biological processes.

Enzyme Mechanism Studies: The significance of D-ribitol-[1-13C] extends to the realm of studying the catalytic mechanisms of enzymes involved in sugar alcohol metabolism. Through the utilization of this isotopically labeled substrate, scientists can conduct comprehensive kinetic and mechanistic investigations to elucidate the intricate workings of enzymes. This knowledge is pivotal for designing enzyme inhibitors or engineering enzymes with specific attributes, driving advancements in enzymology.

Structural Biology: In the domain of structural biology, D-ribitol-[1-13C] emerges as a crucial tool for NMR spectroscopy-based structural analyses of proteins and nucleic acids. The labeled carbon atoms furnish distinctive NMR signals that facilitate the determination of molecular structures and interactions. This application plays a vital role in drug discovery efforts and deepening our understanding of biomolecular assembly processes, offering insights that shape the future of biostructural research.

Diagnostic Research: D-ribitol-[1-13C] is a valuable asset in diagnostic research aimed at investigating metabolic disorders. By administering this labeled compound to patients or experimental models, researchers can monitor its metabolic fate and detect abnormalities within metabolic pathways. This investigative approach aids in the diagnosis and comprehension of conditions like inherited metabolic diseases, paving the way for novel insights into disease mechanisms and potential therapeutic interventions.