D-Arabinose-[1,3-13C2], an isotopically labeled sugar widely utilized in metabolic and biochemical research, showcases its versatility through the key applications:
Metabolic Flux Analysis: Delving into the intricate world of metabolic flux analysis, researchers harness D-Arabinose-[1,3-13C2] to meticulously trace the journey of carbon atoms across metabolic pathways. By scrutinizing the incorporation of the 13C label into diverse metabolic intermediates and end products, scientists unveil the hidden nuances of metabolic fluxes, offering profound insights into cellular metabolism. This method proves instrumental in unraveling the complexities of carbohydrate metabolism and optimizing processes in metabolic engineering with precision and finesse.
NMR Spectroscopy: In the domain of nuclear magnetic resonance (NMR) spectroscopy, the strategic integration of 13C atoms within D-Arabinose-[1,3-13C2] elevates it to a critical tool for structural and dynamic investigations of biomolecules. This isotopic labeling enhances the resolution and sensitivity of NMR experiments, enabling researchers to delve deep into protein-carbohydrate interactions, explore conformational properties, and dissect enzymatic mechanisms with unparalleled accuracy and detail.
Stable Isotope Labeling in Cell Culture: Within the realm of cell culture studies, D-Arabinose-[1,3-13C2] emerges as a potent stable isotope tracer, offering valuable insights into cellular sugar uptake and utilization. Through the analysis of labeled metabolites, scientists unravel the mysteries of sugar transport mechanisms, metabolic pathways, and regulatory processes within cells, laying the groundwork for unraveling the complexities of cell physiology and guiding the development of strategic metabolic interventions with meticulous precision.
Glycobiology Research: As a pivotal reagent in glycobiology, D-Arabinose-[1,3-13C2] empowers researchers to delve deep into the structure, function, and biosynthesis of glycans. Leveraging this isotopically labeled sugar, scientists pinpoint and label specific sugar residues within glycoproteins, glycolipids, and polysaccharides, enabling detailed analysis of glycan structures and their biological significance. This application sheds light on glycan-mediated cell signaling pathways, immune responses, and the intricate world of cellular interactions and signaling mechanisms, revealing the depth of complexity within biological systems.