D-gluconic acid-[1,3-13C2] potassium salt

D-gluconic acid-[1,3-13C2] potassium salt, a stable isotope-labeled compound, finds utility across diverse scientific disciplines for precise measurements and analyses. Here are the key applications of D-gluconic acid-[1,3-13C2] potassium salt:

Metabolic Flux Analysis: Acting as a pivotal tool in unraveling metabolic pathways within microbial and mammalian cells, D-gluconic acid-[1,3-13C2] potassium salt allows researchers to trace the intricate flow of carbon atoms through these networks. By integrating this labeled compound, scientists can quantitatively gauge the dynamic carbon fluxes, shedding light on the intricacies of cellular metabolism and facilitating the optimization of metabolic engineering applications.

Stable Isotope Labeling: In the realms of nutritional and biomedical research, D-gluconic acid-[1,3-13C2] potassium salt serves as a crucial stable isotope tracer. This compound aids in delineating glucose metabolic pathways in vivo by meticulously tracking its conversion and integration into biochemical processes. Such studies offer invaluable insights into the underlying mechanisms of diseases like diabetes and various metabolic disorders, enriching our understanding of physiological processes.

Analytical Chemistry: Esteemed for its significance in mass spectrometry, this compound enables the precise quantification of gluconic acid and its metabolites. The inclusion of the labeled molecule allows for the distinction and accurate measurement of analytes within complex biological samples. This advancement not only bolsters the reliability and sensitivity of analytical methods but also enhances their utility in metabolomics and pharmacokinetics, driving innovation in analytical chemistry.

Enzymology: Leveraging D-gluconic acid-[1,3-13C2] potassium salt, researchers delve into the realm of enzyme kinetics and mechanisms associated with gluconate dehydrogenases. By meticulously tracking the journey of labeled carbon atoms during enzymatic reactions, scientists unveil the intricate pathways and activity of these enzymes. This comprehensive understanding aids in characterizing enzyme function and catalyzes the development of enzyme-centric applications within the realm of biotechnology, underscoring the pivotal role of stable isotope labeling in advancing enzymology.