D-fructose-[1,3,4,6-13C4]

D-fructose-[1,3,4,6-13C4], a stable isotope-labeled compound, is extensively utilized in metabolic and biochemical research. Here are the key applications of D-fructose-[1,3,4,6-13C4]:

Metabolic Flux Analysis: Delving into cell metabolism, researchers leverage D-fructose-[1,3,4,6-13C4] to trace carbon pathways. By scrutinizing the distribution of labeled carbon atoms in metabolic products, scientists can quantify metabolic fluxes and gain intricate insights into cellular metabolism. This application plays a pivotal role in crafting metabolic engineering strategies and fine-tuning biotechnological processes.

Diagnostic Imaging: In the realm of medical investigation, D-fructose-[1,3,4,6-13C4] finds utility in magnetic resonance imaging (MRI) and positron emission tomography (PET) studies. Through isotopic labeling, precise tracking of sugar metabolism in organs like the liver and brain is made possible. This aids in diagnosing metabolic disorders and monitoring the effectiveness of treatments, offering a nuanced understanding of organ function and abnormalities.

Isotope Dilution Studies: In the domain of isotope dilution experiments, D-fructose-[1,3,4,6-13C4] is instrumental in quantifying fructose concentrations in biological samples. By introducing a known quantity of labeled fructose, researchers can determine the ratio of labeled to unlabeled fructose, facilitating accurate quantification. This method is particularly advantageous in the realms of nutritional studies and metabolic research.

Biological Pathway Elucidation: To unravel intricate biochemical pathways in diverse organisms, researchers employ D-fructose-[1,3,4,6-13C4]. By investigating the metabolism of labeled fructose, scientists can unveil the intermediates and steps within metabolic pathways. This application serves as a cornerstone for advancing knowledge in the fields of biochemistry, genetics, and physiology, shedding light on the complex workings of biological systems.