D-Allose-[2-13C]

D-Allose-[2-13C], a stable isotope-labeled sugar utilized for diverse research applications in bioscience, presents a realm of intriguing possibilities. Here we outline the key applications of D-Allose-[2-13C]:

Metabolic Flux Analysis: Delving into cellular metabolic pathways, D-Allose-[2-13C] unveils a tapestry of insights as researchers track the isotope's integration into various metabolites. This intricate process sheds light on how sugars navigate cellular metabolic networks, providing granular data on crucial metabolic fluxes. Such revelations are pivotal for exploring metabolic disorders and fine-tuning metabolic engineering strategies, shaping the forefront of metabolic research.

Enzyme Mechanism Studies: Embarking on a journey into the depths of carbohydrate metabolism, researchers wield D-Allose-[2-13C] as a beacon to illuminate the mechanisms of pivotal enzymes. By employing isotope-labeled substrates, scientists meticulously trace specific carbon atoms through enzymatic cascades, unraveling the mysteries of enzyme functions, reaction mechanisms, and catalytic efficiencies. This nuanced approach enhances our understanding of enzymatic processes, paving the way for groundbreaking discoveries in carbohydrate biochemistry.

NMR Spectroscopy: Within the realm of Nuclear Magnetic Resonance (NMR) spectroscopy, D-Allose-[2-13C] emerges as a star player, elucidating the structure and dynamics of carbohydrates with finesse. The distinctive spectral signals of the [2-13C] isotope label offer a window into the interactions and conformations of carbohydrate molecules, painting a vivid picture of their molecular dance. This application stands as a cornerstone in deciphering carbohydrate chemistry and unraveling the intricacies of biomolecular interactions, enriching our comprehension of biomolecular structures.

Glycobiology Research: At the nexus of glycobiology exploration, D-Allose-[2-13C] shines as a versatile tool for unraveling the mysteries of glycans. Researchers utilize this labeled sugar to delve into the biosynthesis and modification of glycan structures, tracing its incorporation into key players like glycoproteins and glycolipids. This deep dive into sugar involvement within cellular functions unveils new vistas in understanding glycan roles and heralds advancements in glycan-based therapeutic interventions, propelling the field of glycobiology forward.