D-altrose-[2,3-13C2]

D-altrose-[2,3-13C2], a stable isotope-labeled sugar, finds diverse applications in biochemical and medical research. Here are the key applications:

Metabolic Flux Analysis: Integral to metabolic flux analysis, D-altrose-[2,3-13C2] serves as a tracer for unraveling intricate metabolic pathways and comprehending the flow of carbon atoms in biochemical reactions. By introducing this labeled sugar into cellular systems, scientists can meticulously track its conversion into different metabolites using advanced techniques like mass spectrometry and NMR spectroscopy. This approach aids in unveiling dynamic interactions within metabolic networks and pinpointing critical metabolic bottlenecks.

Glycosylation Studies: In the realm of glycosylation research, D-altrose-[2,3-13C2] plays a crucial role in investigating the integration of specific sugars into glycoproteins and glycolipids. By labeling glycosylated molecules, researchers can dissect glycan structures and their implications in essential biological processes such as cell signaling and immune responses. This insightful analysis sheds light on disease mechanisms and unveils potential targets for therapeutic interventions.

Nutritional Research: Diving into nutritional research, D-altrose-[2,3-13C2] emerges as a valuable tool for exploring the metabolic repercussions of distinct dietary sugars and their isotopic labeling on human health. By integrating this labeled sugar into dietary studies, scientists can monitor its metabolic destiny and evaluate its effects on glucose metabolism, insulin sensitivity, and energy equilibrium. This data is instrumental in enhancing our comprehension of the nutritional impacts and health implications of various sugar types.

Enzyme Mechanism Studies: For studying enzyme mechanisms and kinetics, D-altrose-[2,3-13C2] stands out as an indispensable asset. By leveraging this isotope-labeled sugar as a substrate, scientists can delve deep into how enzymes interact with this sugar and catalyze its conversion into diverse products. This exploration aids in identifying active sites, catalytic residues, and unraveling the nuances of enzyme specificity and functionality, shedding light on the intricate world of enzymatic reactions.