D-arabinose-[2,3,4,5-13C4]

D-arabinose-[2,3,4,5-13C4], a specialized form of D-arabinose with four carbon atoms isotopically labeled with carbon-13, finds diverse applications in scientific research. Here are the key applications of D-arabinose-[2,3,4,5-13C4]:

Metabolic Flux Analysis: Widely utilized in metabolic flux analysis, D-arabinose-[2,3,4,5-13C4] serves as a powerful tool for tracing the carbon flow across different metabolic pathways. By introducing this labeled sugar into cell cultures, researchers can meticulously track how cells metabolize carbohydrates, offering profound insights into the intricacies of cellular metabolism and regulatory mechanisms.

Structural Biology: Leveraging D-arabinose-[2,3,4,5-13C4], researchers delve into the realm of carbohydrate structural analysis using nuclear magnetic resonance (NMR) spectroscopy. The carbon-13 labeling significantly enhances the resolution and sensitivity of NMR signals, enabling detailed structural elucidation critical for unraveling the configuration and conformational dynamics of complex carbohydrates.

Enzyme Mechanism Studies: Scientists employ D-arabinose-[2,3,4,5-13C4] to probe the mechanisms underlying carbohydrate-metabolizing enzymes. By monitoring the isotopic label throughout enzymatic reactions, researchers can discern specific steps and intermediates in the catalytic processes, aiding in the design of inhibitors or modulators with therapeutic potential.

Glycosylation Pathway Analysis: In the realm of glycosylation studies, D-arabinose-[2,3,4,5-13C4] plays a pivotal role in tracing the integration of sugar molecules into glycoproteins. Understanding glycosylation patterns is paramount for characterizing protein function, stability, and interactions, particularly crucial in biopharmaceutical production, where glycosylation patterns significantly impact drug efficacy and safety.