D-glucose-[2,4-13C2]

D-glucose-[2,4-13C2], a stable isotopically labeled form of glucose utilized extensively in scientific research, holds immense significance in various applications. Here are the key applications of D-glucose-[2,4-13C2]:

Metabolic Flux Analysis: In the realm of cellular metabolism, D-glucose-[2,4-13C2] serves as a crucial tool for tracking metabolic pathways and quantifying fluxes. By introducing the labeled glucose into cell cultures, researchers can meticulously trace the distribution of the 13C label across different metabolic intermediates. This method provides in-depth insights into cellular metabolic activities, shedding light on the intricate dynamics of metabolic networks.

Magnetic Resonance Spectroscopy (MRS): Leveraging the isotopic labeling of D-glucose-[2,4-13C2], scientists delve into the realm of MRS studies with a focus on 13C NMR spectroscopy. This approach allows researchers to observe and measure metabolites, offering a window into studying metabolic processes in vivo. Such applications are pivotal in unraveling the mysteries of brain metabolism, cancer metabolism, and other physiological processes, enriching our understanding of complex biological phenomena.

Glycolysis and Gluconeogenesis Studies: Delving into the biochemical pathways of glycolysis and gluconeogenesis, D-glucose-[2,4-13C2] emerges as a key player. By tracking the isotope through these pathways, scientists uncover the roles of specific enzymes and unravel the intricate regulation of metabolic routes. Such insights prove invaluable in investigating metabolic disorders and devising novel therapeutic interventions, paving the way for advancements in medical science.

Isotopic Enrichment Studies: In the realm of isotopic enrichment studies, D-glucose-[2,4-13C2] takes center stage in exploring the fate of glucose carbon atoms within diverse biosynthetic pathways. Researchers delve into how glucose carbon is harnessed in the biosynthesis of crucial components like amino acids, lipids, and nucleotides. These studies are instrumental in unraveling the intricate dance of nutrient utilization and metabolic adaptation in varying physiological and pathological states, providing a deeper understanding of the body's metabolic intricacies.