D-fructose-[3,4-13C2]

D-fructose-[3,4-13C2], a stable isotopically labeled compound, finds wide applications in diverse research fields such as metabolic studies and structural biology. Here are the key applications of D-fructose-[3,4-13C2]:

Metabolic Tracing: Acting as a profound metabolic tracer, D-fructose-[3,4-13C2] enables intricate insights into carbohydrate metabolism through metabolic flux analysis. By meticulously tracking the labeled carbon atoms along metabolic pathways, researchers unveil the dynamic interplay of glucose metabolism and its integration with other metabolic networks, shedding light on the complex metabolic shifts linked to conditions like diabetes and obesity.

Nutritional Research: Within the realm of nutritional investigations, D-fructose-[3,4-13C2] plays a pivotal role in unraveling the metabolic destiny of dietary fructose. Researchers harness this compound to scrutinize the processing and utilization of fructose in the body, delving into its implications on liver function and overall energy equilibrium. These revelations advance our comprehension of the health ramifications of high-fructose diets, offering key insights into metabolic health.

Structural Biology: Serving as an indispensable tool in nuclear magnetic resonance (NMR) spectroscopy and mass spectrometry studies, D-fructose-[3,4-13C2] leverages isotopic labeling to provide distinct signals crucial for resolving the structures and interactions of carbohydrate molecules. This application is fundamental in deciphering the intricate structure-function relationships within glycobiology, unveiling the molecular intricacies governing biological processes.

Isotopic Enrichment Studies: In the realm of biochemistry, D-fructose-[3,4-13C2] finds utility in generating isotopically enriched derivatives tailored for diverse applications. These derivatives serve as invaluable resources for exploring enzyme mechanisms and kinetic isotope effects. Through the incorporation of labeled fructose into other molecules, researchers attain specific labeling patterns that streamline detailed mechanistic investigations, driving forward our understanding of biochemical processes.