D-lyxose-[4-13C]

D-lyxose-[4-13C] is a stable isotope-labeled sugar that has a variety of applications in scientific research. Here are some key applications of D-lyxose-[4-13C]:

Metabolic Flux Analysis: D-lyxose-[4-13C] is utilized in metabolic studies to trace the flow of carbon through metabolic pathways. By tracking the fate of 13C-labeled D-lyxose, researchers can gain insights into the metabolic fluxes and understand how cells utilize this sugar. This is particularly useful in elucidating metabolic mechanisms and optimizing metabolic engineering strategies.

NMR Spectroscopy: In structural biology and biochemistry, D-lyxose-[4-13C] is employed as a probe in NMR spectroscopy to study carbohydrate-protein interactions. The 13C label provides a detectable signal, enabling detailed analysis of the sugar's binding properties and structural conformations. This helps in understanding the molecular basis of carbohydrate recognition and binding.

Biomolecular Labeling: D-lyxose-[4-13C] can be used for labeling biomolecules in order to study biosynthesis and degradation processes. By incorporating the labeled sugar into biological systems, researchers can track its integration into macromolecules such as nucleic acids and polysaccharides. This provides valuable information on the dynamics and turnover of these biomolecules.

Physiological Studies: In physiological and nutritional research, D-lyxose-[4-13C] serves as a tracer to study the absorption, distribution, and metabolism of pentose sugars in vivo. By administering the labeled compound to model organisms or human subjects, scientists can monitor its kinetics and metabolic fate. This application is critical for understanding sugar metabolism disorders and assessing dietary interventions.