D-fructose-[4,5-13C2]

D-fructose-[4,5-13C2], a carbon-labeled sugar molecule utilized in various scientific investigations, serves as a valuable tool for tracing metabolic pathways and analyzing biochemical processes. Here are the key applications of D-fructose-[4,5-13C2]:

Metabolic Flux Analysis: Within the realm of metabolic flux analysis, D-fructose-[4,5-13C2] plays a pivotal role in unraveling the intricate flow of carbon atoms across diverse metabolic pathways. By integrating this labeled fructose into cellular systems, researchers can meticulously track the distribution of the isotopic label using cutting-edge techniques such as NMR or mass spectrometry. This approach enables the meticulous mapping of metabolic pathways and offers profound insights into cellular metabolism at a granular level.

Nutritional Studies: In the realm of nutritional research, D-fructose-[4,5-13C2] serves as a powerful tool for investigating the metabolism and absorption of fructose within the human body. Through the administration of labeled fructose to test subjects, scientists can quantitatively assess its conversion into various metabolites and its involvement in metabolic disorders. This application is essential for comprehending the intricate interactions between dietary fructose consumption and health conditions such as obesity and diabetes, shedding light on the complex interplay between nutrition and physiology.

Enzyme Mechanism Investigation: D-fructose-[4,5-13C2] emerges as a valuable asset in the realm of studying enzymatic processes implicated in carbohydrate metabolism. By leveraging this labeled substrate, researchers can delve into the specificity, kinetics, and mechanism of enzymes such as isomerases and aldolases with unparalleled precision. This profound understanding of enzyme function is instrumental in the development of novel enzyme inhibitors and the design of targeted therapies for metabolic diseases, driving innovation in the field of biomedicine.

Biotechnological Applications: Within the domain of industrial biotechnology, D-fructose-[4,5-13C2] stands out as a versatile tool for optimizing fermentation processes and enhancing product yields. Through tracking the fate of labeled carbon atoms in microbial systems, scientists can fine-tune the efficiency of bioprocesses responsible for producing biofuels, pharmaceuticals, or other valuable products. This strategic utilization of labeled fructose contributes to the advancement of robust and sustainable biotechnological applications, offering a pathway towards greener industrial practices and enhanced product development.