D-glucitol-[4,6-13C2]

D-glucitol-[4,6-13C2], also known as isotopically labeled D-sorbitol, stands as a carbon-labeled sugar alcohol with profound implications in various biochemical and clinical research endeavors. Here are the key applications of D-glucitol-[4,6-13C2]:

Metabolic Flux Analysis: Scientists harness D-glucitol-[4,6-13C2] to track and scrutinize metabolic pathways within prokaryotic and eukaryotic cells. By monitoring the incorporation and transfer of labeled carbons across the metabolic network, researchers illuminate metabolic flux distributions and pinpoint pivotal regulatory nodes within these pathways.

NMR Spectroscopy: Employing 13C-labeled D-glucitol in nuclear magnetic resonance (NMR) spectroscopy, researchers delve into carbohydrate metabolism and structural biology. By following the trajectory of labeled carbons, scientists glean intricate insights into the dynamics and architecture of complex carbohydrates and their interactions with other biomolecules, shedding light on the molecular underpinnings of diseases and the design of targeted therapies.

Biomedical Research: In studies exploring the transport and utilization of sorbitol in tissues, particularly in diabetic contexts, D-glucitol-[4,6-13C2] plays a crucial role. Its labeled form facilitates precise tracking in both in vivo and in vitro experiments, aiding in unraveling the involvement of sorbitol in diabetic complications like neuropathy and retinopathy.

Stable Isotope-Labeling: Within proteomics and metabolomics realms, D-glucitol-[4,6-13C2] serves as a stable isotope-labeled internal standard for precise quantitative analyses. Its utilization enables accurate quantification of sugar alcohols and related compounds in biological samples, ensuring dependable data for high-precision investigations such as those focusing on metabolic diseases and biomarker discovery.