D-Ribitol-[5-13C]

D-Ribitol-[5-13C], a labeled compound utilized in diverse scientific research endeavors to map metabolic pathways and investigate biochemical processes. Below are the key applications of D-Ribitol-[5-13C]:

Metabolic Flux Analysis: Employing D-Ribitol-[5-13C] in metabolic flux analysis (MFA) enables researchers to track the journey of carbon atoms through intricate metabolic networks. Integration of this labeled compound into cellular metabolic processes allows for the quantification of metabolic fluxes, shedding light on how cells distribute resources. This knowledge plays a crucial role in metabolic engineering efforts and the optimization of microbial production systems.

NMR Spectroscopy: Within the realm of research utilizing nuclear magnetic resonance (NMR) spectroscopy, D-Ribitol-[5-13C] stands as a tracer revealing the structure and dynamics of metabolic intermediates. The 13C label emits a unique NMR signal, facilitating in-depth exploration of metabolic pathways. Researchers gain valuable insights into reaction mechanisms and the dynamic functions of enzymes through this investigative approach.

Isotope Labeling Studies: D-Ribitol-[5-13C] finds application in isotope labeling studies aimed at unraveling the biosynthesis and degradation pathways of ribitol-containing biomolecules. Tracking the incorporation of the 13C label allows scientists to pinpoint metabolic intermediates and elucidate complex biochemical pathways. This tool proves invaluable in both fundamental scientific exploration and practical bioscience applications.

Quantitative Proteomics: In the domain of quantitative proteomics, D-Ribitol-[5-13C] contributes to stable isotope labeling by amino acids in cell culture (SILAC) experiments. This method enables precise quantification of proteins under varying biological conditions, utilizing the 13C label as a reference standard. By enhancing the reliability and sensitivity of proteomic analyses, this technique offers deeper insights into protein functionality and regulation.