D-Glucose-[5-18O]

D-Glucose-[5-18O] is a stable isotope-labeled compound used extensively in biochemical and metabolic studies. Here are some key applications of D-Glucose-[5-18O]:

Metabolic Flux Analysis: Researchers use D-Glucose-[5-18O] to trace metabolic pathways and measure fluxes in cellular systems. By monitoring the incorporation of the stable isotope into metabolic intermediates, scientists can gain insights into glucose utilization and metabolic enzyme activities. This application is crucial for understanding diseases like diabetes and for developing metabolic engineering strategies.

Positron Emission Tomography (PET) Imaging: D-Glucose-[5-18O] can be utilized in PET imaging to study glucose metabolism in vivo. This allows researchers to visualize and quantify glucose uptake in tissues, which is especially valuable in cancer research for identifying hypermetabolic tumors. PET imaging with D-Glucose-[5-18O] provides a non-invasive method to assess metabolic activity and monitor disease progression or treatment responses.

Enzymatic Activity Studies: In enzymology, D-Glucose-[5-18O] aids in examining the action of enzymes involved in glucose metabolism. By using the labeled glucose, scientists can study enzyme kinetics and mechanisms with high precision. This is beneficial for drug development, as it helps in identifying enzyme inhibitors or activators.

Pharmaceutical R&D: D-Glucose-[5-18O] serves as a tracer in the development of pharmaceuticals, particularly those targeting metabolic diseases. Researchers use the labeled compound to study drug metabolism and pharmacokinetics. This helps in understanding how a drug interacts with metabolic pathways, aiding in the optimization of therapeutic agents.