D-fructose-[5,6-13C2]

D-fructose-[5,6-13C2], an isotopic compound with labeled carbon atoms, is a valuable tool in research for tracing metabolic pathways and investigating biological processes. Here are the key applications of D-fructose-[5,6-13C2]:

Metabolic Flux Analysis: Crucial for metabolic flux studies, D-fructose-[5,6-13C2] is utilized to monitor the incorporation of labeled carbon atoms into various metabolites. By examining the isotopic distribution, scientists can quantitatively assess metabolic reaction rates and comprehend the dynamics of metabolic networks. This technique plays a pivotal role in exploring metabolic disorders and optimizing strategies for metabolic engineering, shedding light on the intricacies of cellular metabolism.

NMR Spectroscopy: In the realm of nuclear magnetic resonance (NMR) spectroscopy, D-fructose-[5,6-13C2] emerges as a key player for detailed structural analysis of carbohydrates and their interactions within biological systems. The introduction of 13C labeling enhances the visibility of carbon atoms in fructose, providing precise insights into molecular conformation and dynamics. This application is indispensable in structural biology and the development of carbohydrate-based drugs, offering a deeper understanding of molecular structures and interactions.

Positron Emission Tomography (PET): Delving into the realm of PET imaging, D-fructose-[5,6-13C2] serves as a tracer to investigate glucose metabolism in tissues. By monitoring the uptake and utilization of labeled fructose, researchers can glean valuable information on metabolic activity in various organs, particularly in cancerous tissues characterized by altered glucose metabolism. This approach aids in diagnosing and tracking metabolic disorders and cancer progression, offering non-invasive insights into tissue-specific metabolism dynamics.

Nutritional Studies: Playing a pivotal role in nutritional research, D-fructose-[5,6-13C2] facilitates the exploration of fructose metabolism in the human body. By tracing the pathways of labeled fructose, scientists can unravel its impact on health outcomes, such as its implications in obesity, diabetes, and cardiovascular diseases. This knowledge is essential for crafting evidence-based dietary guidelines and interventions aimed at enhancing metabolic health and disease prevention, offering a deeper understanding of the interplay between diet and physiological well-being.