D-glucitol-[5,6-13C2]

D-glucitol-[5,6-13C2], an isotopically labeled compound utilized in diverse bioscience research domains, boasts a myriad of applications. Here are the key applications of D-glucitol-[5,6-13C2]:

Metabolic Flux Analysis: In the realm of stable isotope labeling experiments, D-glucitol-[5,6-13C2] emerges as a valuable tool for unraveling the intricate carbon flow within metabolic pathways. By scrutinizing the distribution of the 13C label across metabolic intermediates and end-products, researchers can glean profound insights into cellular metabolic fluxes. This technique stands as a cornerstone for deciphering metabolic regulation and refining strategies for metabolic engineering, shedding light on the dynamic nature of cellular metabolism.

NMR Spectroscopy: The strategic incorporation of 13C labeling in D-glucitol-[5,6-13C2] elevates its significance in nuclear magnetic resonance (NMR) spectroscopy investigations. Enriched with distinguishable carbon atoms, this compound furnishes distinctive NMR signals, facilitating in-depth structural and conformational analyses of carbohydrates and related biomolecules. This application plays a pivotal role in unraveling molecular interactions and dynamics within complex biological systems, providing a comprehensive understanding of molecular architectures and functions.

Glycosylation Studies: In the domain of glycosylation research, D-glucitol-[5,6-13C2] emerges as a potent probe for scrutinizing the assimilation of labeled sugars into glycoproteins and glycolipids. By meticulously tracing the 13C label, scientists can delve into the biosynthesis and turnover dynamics of glycoconjugates in cellular contexts. This knowledge serves as a linchpin for unraveling the critical roles of glycosylation in cellular processes and disease pathologies, illuminating the intricacies of cellular glycosylation mechanisms and their implications in health and disease.

Mass Spectrometry: Within the realm of mass spectrometry-based metabolomics, D-glucitol-[5,6-13C2] assumes a pivotal role as an internal standard and tracer, bolstering the accuracy and precision of metabolic profiling endeavors. The unique mass shift induced by the 13C atoms facilitates precise detection and quantification of metabolites within complex biological samples, enhancing the sensitivity and specificity of metabolic analyses. This application stands at the forefront of biomarker discovery and metabolic profiling, offering insights into metabolic perturbations associated with health and disease states, paving the way for targeted diagnostic and therapeutic interventions.