D-Glucose-[6-18O]

D-Glucose-[6-18O] is a labeled form of glucose used primarily in research to trace metabolic pathways and study biochemical processes. Here are some key applications of D-Glucose-[6-18O]:

Metabolic Flux Analysis: D-Glucose-[6-18O] is used to trace the metabolic pathways in cells by following the incorporation of the 18O isotope. This helps researchers understand how glucose is metabolized and the regulation of metabolic fluxes. It is particularly useful in studying the dynamics of glycolysis and the pentose phosphate pathway.

Stable Isotope Labeling: In proteomics and metabolomics, D-Glucose-[6-18O] serves as a stable isotope label to quantify and track metabolic changes in cells and tissues. By incorporating this labeled glucose into cell cultures, it is possible to measure metabolic rates and investigate alterations in response to different stimuli or disease states. This contributes to a deeper understanding of cellular metabolism and metabolic disorders.

Brain Imaging Studies: D-Glucose-[6-18O] can be used in conjunction with imaging techniques such as positron emission tomography (PET) to study glucose metabolism in the brain. The labeled glucose allows for the observation and measurement of brain activity and energy consumption in real time. This is valuable in research on neurological disorders, cognitive function, and brain aging.

Diabetes Research: In diabetes research, D-Glucose-[6-18O] is employed to investigate glucose utilization and insulin sensitivity. By tracing the labeled glucose, scientists can better understand how glucose uptake and metabolism are affected in diabetic conditions. This aids in the development of new therapeutic strategies and the improvement of existing treatments for diabetes management.