D-Alanine-N-T-BOC-[15N]

D-Alanine-N-T-BOC-[15N], a stable isotope-labeled amino acid derivative, finds utility in diverse advanced scientific applications. Here are the key applications of D-Alanine-N-T-BOC-[15N]:

NMR Spectroscopy: Employing D-Alanine-N-T-BOC-[15N] in nuclear magnetic resonance (NMR) spectroscopy facilitates the examination of protein structure and dynamics. The inclusion of the 15N isotope furnishes crucial insights into nitrogen-containing functional groups and their intricate molecular interactions. This application empowers researchers to delve into protein folding, conformational alterations, and molecular interactions, unraveling the intricacies of biomolecular behavior.

Metabolic Tracing: The application of D-Alanine-N-T-BOC-[15N] in metabolic pathway analysis through stable isotope tracing enables researchers to explore metabolic networks with precision. By introducing this labeled compound into biological systems, researchers can meticulously track its integration and metamorphosis within metabolic pathways. This technique plays a pivotal role in unraveling convoluted metabolic fluxes and illuminating the landscape of cellular metabolism, offering profound insights into cellular energetics.

Mass Spectrometry: In the realm of mass spectrometry-based proteomics, D-Alanine-N-T-BOC-[15N] emerges as an indispensable internal standard and labeling reagent. Its deployment facilitates accurate quantitative analysis of peptides and proteins by endowing them with a distinctive isotopic signature. This application enhances the precision and robustness of proteomic investigations, enriching biomarker discovery endeavors and bolstering the field of functional proteomics through nuanced proteome characterization.

Biochemical Synthesis: D-Alanine-N-T-BOC-[15N] plays a pivotal role in the synthesis of labeled peptides and proteins for diverse research pursuits. Integrating 15N-labeled alanine into synthetic peptides enables researchers to elucidate their biochemical attributes and molecular interactions with clarity. These labeled biomolecules represent valuable assets in structural biology and enzymology studies, fostering a deeper comprehension of biological macromolecules and their intricate functions in living systems.