D-Proline-[1-13C]

D-Proline-[1-13C], a stable isotope-labeled version of the amino acid proline, finds extensive use across diverse scientific disciplines. Explore below the high perplexity and burstiness of its applications in key fields:

NMR Spectroscopy: Diving into nuclear magnetic resonance (NMR) spectroscopy, researchers leverage D-Proline-[1-13C] to delve into the structural intricacies of proteins and peptides. The incorporation of the 13C label amplifies signal clarity, offering a detailed peek into molecular structure and dynamics. This application serves as a cornerstone for unraveling protein folding nuances, conformational shifts, and interactions with surrounding molecules.

Metabolic Flux Analysis: In the realm of metabolic investigations, D-Proline-[1-13C] acts as a potent tracer to unfurl the mysteries of cellular metabolic pathways and fluxes. By monitoring the journey of the 13C label through metabolic intermediates, scientists glean insights into amino acid consumption and biosynthetic routes. Such knowledge is indispensable for deciphering metabolic control mechanisms and fine-tuning metabolic engineering schemes.

Protein Structure-Function Studies: Delving into the intricate dance between protein structure and function, researchers employ D-Proline-[1-13C] to map out the roles of proline residues within proteins. The 13C label aids in precise localization of proline residues, shedding light on their impact on protein stability, folding dynamics, and functional prowess. This application is pivotal in the quest to design proteins with augmented functionalities or novel properties.

Pharmaceutical Development: Within the realm of pharmaceutical innovation, D-Proline-[1-13C] emerges as a crucial player in the realm of drug metabolism studies. By acting as a diagnostic tool, the labeled proline facilitates comprehension of a drug's pharmacokinetics and metabolic destiny within the organism. This application guides decisions in drug formulation, dosing strategies, and safety evaluations throughout the drug development journey.