D-talose-[UL-13C6]

D-talose-[UL-13C6], a universally labeled isotope of the rare sugar D-talose, finds primary use in biochemical research. Here are the key applications of D-talose-[UL-13C6]:

Metabolic Flux Analysis: Employing D-talose-[UL-13C6] in metabolic flux analysis, researchers delve into the intricate pathways of carbohydrates. By tracking the incorporation and turnover of this labeled sugar within cellular metabolic networks, scientists unravel the dynamic interplay of carbohydrate metabolism and the intricate regulation of metabolic pathways, shedding light on the complexities of biological processes.

Structural Biology: In the realm of structural biology, D-talose-[UL-13C6] serves as a valuable tool for exploring the structural and functional aspects of carbohydrate-binding proteins. The labeled sugar facilitates cutting-edge NMR and mass spectrometry analyses, unveiling binding sites and detecting conformational changes. This critical information deepens our understanding of protein-ligand interactions, leading to the design of precision inhibitors or modulators with potential therapeutic implications.

Stable Isotope Labeling: Serving as a benchmark in quantitative proteomics and metabolomics, D-talose-[UL-13C6] acts as a stable isotope standard, ensuring the precision of measurements across various biological samples. By integrating this compound into experimental protocols, researchers elevate the accuracy and reproducibility of omics studies, paving the way for robust conclusions and insights into complex biological systems.

Enzyme Kinetics: Integral to enzyme kinetics, D-talose-[UL-13C6] plays a pivotal role in deciphering the catalytic mechanisms of enzymes involved in carbohydrate metabolism. Through meticulous tracing of the labeled substrate, scientists quantify reaction rates, delineate kinetic parameters, and unveil potential regulatory sites, delineating a pathway towards enzyme engineering and the development of innovative biotechnological applications.