DL-Alanine-[2-13C,2-d]

DL-Alanine-[2-13C,2-d], an isotopically labeled derivative of the amino acid alanine, finds versatile applications in research and analytical endeavors. Here are key applications of DL-Alanine-[2-13C,2-d] presented with a high degree of perplexity and burstiness:

Metabolic Flux Analysis: In the intricate realm of metabolic flux analysis, DL-Alanine-[2-13C,2-d] emerges as a crucial tool for deciphering the intricate carbon atom pathways within metabolic networks. By introducing this specially labeled alanine into biological systems, researchers embark on a journey to trace the intricate redistribution of labeled carbon in metabolic intermediates. This analytical approach serves as a guiding light, unraveling the mysteries of metabolic pathways and their intricate regulation, offering profound insights into the complex landscape of cellular metabolism.

NMR Spectroscopy: Elevating the realm of nuclear magnetic resonance (NMR) spectroscopy to new heights, DL-Alanine-[2-13C,2-d] acts as a beacon for delving into enzyme mechanisms and protein structures. The isotopic labels woven into its molecular fabric illuminate distinct signals in NMR spectra, enabling scientists to conduct a meticulous analysis of molecular interactions and dynamics. This gateway allows researchers to delve deeper into the realm of protein-ligand binding and conformational changes, shedding light on the intricate dance of biomolecular interactions.

Stable Isotope Tracing: Positioned at the cutting edge of stable isotope tracing experiments, DL-Alanine-[2-13C,2-d] serves as a cornerstone for studies delving into amino acid metabolism and protein synthesis. The dual isotope labeling embedded within its structure facilitates precise quantification and tracking of alanine's journey into proteins and other metabolic products. This technique serves as a crucial beacon in the realm of nutritional biochemistry, offering a lens through which scientists can explore disease-related metabolic alterations, illuminating previously unseen pathways and connections.

Research in Bioenergetics: At the forefront of research into cellular bioenergetics, DL-Alanine-[2-13C,2-d] plays a pivotal role in assessing metabolic states and unraveling energy production pathways. By closely monitoring its metamorphosis through catabolic and anabolic reactions, researchers embark on a journey to unveil the enigmatic energy balance and efficiency within cellular realms. This invaluable information serves as a cornerstone for decoding the intricate dynamics of cellular energy flow, laying the groundwork for interventions targeted at tackling metabolic disorders and optimizing cellular energy homeostasis.