DL-Glutamic acid-[13C5]

DL-Glutamic acid-[13C5], a stable isotope-labeled compound, finds diverse applications in research and clinical investigations. Here are key applications presented with high perplexity and burstiness:

Metabolic Flux Analysis: Researchers employ DL-Glutamic acid-[13C5] in metabolic flux analysis to scrutinize the flow of carbon atoms within metabolic pathways. By tracing the integration of the ^13C-labeled glutamic acid into intermediates, scientists can quantitatively assess the activity of metabolic pathways. This analysis is fundamental for comprehending cellular metabolism dynamics and identifying potential targets for metabolic engineering.

NMR Spectroscopy: Within nuclear magnetic resonance (NMR) spectroscopy, DL-Glutamic acid-[13C5] serves as a reference compound for investigating protein and enzyme structure. The ^13C labeling enables detailed examination of the chemical milieu surrounding glutamic acid residues. This aids in unveiling protein folding kinetics and interactions in biochemical research, shedding light on intricate molecular dynamics.

Tracer Studies in Clinical Nutrition: Employed as a tracer in clinical nutrition investigations, DL-Glutamic acid-[13C5] contributes insights into amino acid metabolism and protein turnover in human subjects. By administering the labeled compound, researchers can monitor its metabolic trajectory and glean understanding into nutritional status and the impacts of dietary interventions. This knowledge is instrumental for formulating therapeutic diets and managing metabolic disorders effectively.

Biochemical Pathway Elucidation: Utilizing DL-Glutamic acid-[13C5], researchers delve into specific biochemical pathways in cellular models. By analyzing the distribution of the ^13C label among metabolic intermediates, scientists can chart enzymatic reactions and pathway bifurcation points. This exploration aids in unraveling intricate biochemical networks and understanding the regulation of metabolic processes comprehensively.