DL-Histidine-[alpha-15N] 2HCl

DL-Histidine-[alpha-15N] 2HCl, a compound labeled with stable isotopes, finds diverse applications in scientific research. Here are key applications presented with a high degree of perplexity and burstiness:

Protein Structure and Dynamics: Employed in nuclear magnetic resonance (NMR) spectroscopy for studying protein dynamics and structure, DL-Histidine-[alpha-15N] 2HCl plays a vital role. By integrating this specifically labeled histidine into proteins, researchers gain intricate insights into the environments and interactions of histidine residues. This aids in unraveling the complexities of protein folding, conformational alterations, and binding interactions with other molecules.

Metabolic Tracing: In the realm of metabolic research, DL-Histidine-[alpha-15N] 2HCl acts as a tracer to investigate nitrogen metabolism pathways. By tracing the movement of the 15N isotope through metabolic processes, scientists can discern and quantify nitrogen-containing metabolites. This application is pivotal for comprehending amino acid metabolism, biosynthesis pathways, and the regulation of nitrogen balance within cells.

Nutritional Studies: Delving into histidine's role and metabolism in humans and animals, DL-Histidine-[alpha-15N] 2HCl is a crucial component of nutritional studies. By administering this labeled compound, researchers can track histidine absorption, utilization, and excretion. This data is invaluable for understanding dietary needs, protein nutrition, and the repercussions of histidine deficiency on overall health.

Peptide and Protein Synthesis: Within the realm of synthetic chemistry, DL-Histidine-[alpha-15N] 2HCl is integrated into peptides and proteins for specific experimental objectives. The utilization of the 15N-labeled histidine enables precise analysis in mass spectrometry investigations. This capability is instrumental in validating peptide sequences, exploring protein-protein interactions, and formulating new biochemical assays.