DL-Proline-[d7]

DL-Proline-[d7] is a stable isotope-labeled amino acid that is commonly used in various scientific research applications. Here are some key applications of DL-Proline-[d7]:

Metabolomics: DL-Proline-[d7] is used in metabolomics studies to track and quantify metabolic pathways. By incorporating the labeled proline into biological systems, researchers can trace its conversion and use through different metabolic pathways. This application helps in understanding complex metabolic networks and identifying key regulatory points in cellular metabolism.

Protein Structure Analysis: In structural biology, DL-Proline-[d7] aids in the study of protein folding and conformational changes. By substituting specific amino acids with their labeled counterparts, researchers can use nuclear magnetic resonance (NMR) spectroscopy to observe changes in protein structures. This is crucial for elucidating mechanisms of protein function and stability.

Pharmacokinetics: DL-Proline-[d7] can be used in pharmacokinetic studies to understand the absorption, distribution, metabolism, and excretion (ADME) of drugs. When incorporated into drug molecules, the labeled proline helps in tracing the drug’s path through the body. This information is essential for optimizing drug dosage and improving therapeutic efficacy.

Cell Culture Studies: DL-Proline-[d7] is employed in cell culture experiments to study amino acid utilization and protein synthesis in cells. By providing a labeled nutrient source, researchers can monitor the uptake and incorporation of proline into newly synthesized proteins. This application is useful for investigating cellular physiology and the effects of different culture conditions on protein metabolism.