DL-Serine-[13C3,15N]

DL-Serine-[13C3,15N] is an isotopically labeled amino acid often used in various research applications due to its enriched carbon-13 and nitrogen-15 atoms. Here are some key applications of DL-Serine-[13C3,15N]:

Metabolic Flux Analysis: DL-Serine-[13C3,15N] is instrumental in studying metabolic pathways by tracing its incorporation into cellular metabolites. Researchers can track the labeled isotopes through metabolic networks to determine the fluxes and rates of biochemical reactions. This aids in understanding metabolic dynamics and identifying key regulatory checkpoints.

Protein Structure Elucidation: In structural biology, DL-Serine-[13C3,15N] is used in protein NMR (nuclear magnetic resonance) spectroscopy. The labeled serine allows for detailed examination of protein structures, dynamics, and interactions at an atomic level. This information is crucial for drug design and understanding protein function.

Nutritional Studies: DL-Serine-[13C3,15N] can be used to investigate amino acid uptake and metabolism in nutritional science. By administering the labeled amino acid, researchers can study how it is metabolized and utilized within the body. This is valuable for developing dietary interventions and understanding metabolism under different physiological conditions.

Cell Culture Experiments: In cell biology, DL-Serine-[13C3,15N] is used to assess serine’s role in cell growth and function. By incorporating the labeled amino acid into cell culture media, scientists can monitor its utilization and metabolic fate within the cells. This application provides insights into amino acid metabolism and its impact on cell physiology.