DL-Serine-[15N]

DL-Serine-[15N] is an isotopically labeled amino acid used in a variety of biological and chemical research applications. Here are some key applications of DL-Serine-[15N]:

Metabolic Flux Analysis: DL-Serine-[15N] is used in metabolic flux analysis to trace nitrogen incorporation into metabolic pathways. By monitoring the distribution of the [15N] label, researchers can elucidate the metabolic fates and transformations of serine within cells. This helps in understanding serine metabolism and its role in cellular functions.

Protein Structure and Dynamics: Isotopically labeled DL-Serine-[15N] is employed in Nuclear Magnetic Resonance (NMR) spectroscopy to study the structure and dynamics of proteins. The [15N] label provides distinct NMR signals that facilitate the detailed analysis of protein folding, interactions, and conformational changes. This information is crucial for understanding protein function and designing therapeutic interventions.

Biochemical Pathway Elucidation: In biochemical research, DL-Serine-[15N] is used to trace and quantify the involvement of serine in various biosynthetic pathways. By incorporating the labeled serine into cells, scientists can follow its conversion into other amino acids, nucleotides, or other biomolecules. This approach aids in identifying novel biochemical pathways and understanding the regulation of serine metabolism.

In Vivo Labeling Studies: DL-Serine-[15N] is used in in vivo experiments to study nutrient utilization, metabolic integration, and protein turnover. By administering the labeled compound to organisms, researchers can track its incorporation into tissues and organs, providing insights into systemic metabolic responses. This is particularly valuable in understanding disease mechanisms and evaluating the effects of nutritional interventions.