DL-valine-[2-13C]

DL-valine-[2-13C] is a stable isotope-labeled amino acid that is extensively used in bioscience research. Here are some key applications of DL-valine-[2-13C]:

Metabolic Flux Analysis: DL-valine-[2-13C] is employed in metabolic flux analysis to study metabolic pathways and fluxes in cells. By incorporating the labeled valine into metabolic processes, researchers can trace its movement through various biochemical pathways. This allows for a detailed understanding of cellular metabolism and helps identify bottlenecks or key control points within metabolic networks.

Protein Structure and Function Studies: DL-valine-[2-13C] can be used in nuclear magnetic resonance (NMR) spectroscopy to investigate protein structure and dynamics. The 13C label provides specific signals that can be detected and analyzed, aiding in the elucidation of protein folding, interactions, and conformational changes. This application is crucial for understanding protein function and designing drugs that target specific protein structures.

Nutritional Research: In nutritional studies, DL-valine-[2-13C] helps in understanding amino acid metabolism and utilization in the body. By tracking the labeled valine in animal or human subjects, researchers can assess how efficiently it is metabolized and its role in protein synthesis. This information is valuable for developing nutritional supplements and optimizing dietary protocols.

Pharmaceutical Development: DL-valine-[2-13C] is utilized in pharmacokinetic studies to monitor the absorption, distribution, metabolism, and excretion (ADME) of drugs that incorporate valine. The labeled isotope acts as a tracer, providing precise information on drug dynamics within the body. This data is critical for determining optimal dosing regimens and ensuring drug safety and efficacy.