Dothiepin-[d6] HCl (N,N-dimethyl-[d6]) (cis/trans mixture)

Dothiepin-[d6] HCl (NN-dimethyl-[d6]) (cistrans mixture), is a deuterated form of the tricyclic antidepressant dothiepin. Here are some key applications of Dothiepin-[d6] HCl:

Pharmacokinetic Studies: Dothiepin-[d6] HCl is used in pharmacokinetic studies to understand the metabolism and distribution of dothiepin in the body. Its deuterated nature allows for precise tracking using mass spectrometry, distinguishing it from the non-deuterated compound. This aids in the accurate measurement of drug absorption, elimination, and bioavailability.

Drug Metabolism Research: Researchers employ Dothiepin-[d6] HCl to study the metabolic pathways and enzymes involved in the breakdown of dothiepin. By comparing the metabolic profiles of the deuterated and non-deuterated forms, scientists can identify specific enzymes and intermediates in the process. This contributes to a better understanding of drug interactions and potential metabolic issues.

Bioavailability Studies: In clinical research, Dothiepin-[d6] HCl is used to compare the bioavailability of different formulations of dothiepin. By administering deuterated and non-deuterated versions of the drug, researchers can assess the impact of various pharmaceutical formulations on drug absorption and efficacy. This information is crucial for optimizing drug delivery systems.

Stable Isotope Labeling: Dothiepin-[d6] HCl serves as a stable isotope-labeled internal standard in quantitative analysis of dothiepin levels in biological samples. Its use helps in enhancing the accuracy and precision of analytical methods such as liquid chromatography-mass spectrometry (LC-MS). This is important for therapeutic drug monitoring and pharmacokinetic profiling.