α-Ketoglutaric acid-[13C4] disodium salt

α-Ketoglutaric acid-[13C4] disodium salt, a stable isotope-labeled compound utilized across diverse scientific disciplines for detailed metabolic and biochemical analyses. Here are key applications of α-Ketoglutaric acid-[13C4] disodium salt, presented with high perplexity and burstiness:

Metabolic Flux Analysis: Delving into metabolic intricacies, α-Ketoglutaric acid-[13C4] disodium salt serves as a vital tracer in metabolic investigations, shedding light on the TCA cycle and interconnected metabolic pathways. By integrating this isotope into metabolic inquiries, researchers can meticulously monitor carbon flux within cellular metabolism, unraveling metabolic adaptations in varying physiological states and disease contexts.

NMR Spectroscopy: In the domain of NMR spectroscopy, α-Ketoglutaric acid-[13C4] disodium salt plays a pivotal role in exploring enzyme-catalyzed reactions and evaluating metabolic transformations. The labeled carbon imparts a distinctive signal, facilitating the resolution of complex spectra and enabling precise identification of metabolic intermediates. Such granular insights are indispensable for unraveling the mechanistic facets of enzyme functionality.

Nutritional Studies: Foraying into nutritional biochemistry, α-Ketoglutaric acid-[13C4] disodium salt is enlisted to scrutinize amino acid metabolism and nitrogen balance. By tracing its conversion into diverse amino acids, scientists can gauge nutritional values and protein utilization across varied diets. This application proves particularly valuable for optimizing nutritional supplements and unraveling metabolic perturbations linked to amino acid metabolism.

Pharmacokinetic Studies: A cornerstone in pharmacokinetic research, this compound proves invaluable for assessing drug interactions and metabolic transformations within living organisms. Incorporating stable isotopes like [13C4] offers a precise means to track drug metabolites and assess diverse metabolic pathways. Insights gleaned from these studies inform the design of more efficacious therapeutic interventions and enhance our comprehension of drug metabolism.