L-Alanine-[1-13C,3,3,3-d3]

L-Alanine-[1-13C,3,3,3-d3], a stable isotope-labeled amino acid with a myriad of applications in bioscience, offers intricate possibilities. Here are key applications of L-Alanine-[1-13C,3,3,3-d3] presented with a high degree of perplexity and burstiness:

Metabolic Flux Analysis: Delving into the intricate cellular metabolic pathways, L-Alanine-[1-13C,3,3,3-d3] assumes a pivotal role in metabolic flux analysis by meticulously tracking and quantifying metabolic fluxes. Introducing this labeled compound into cell cultures, researchers can scrutinize the assimilation and transformation of labeled carbon, shedding light on the dynamic complexities of metabolic processes. This profound understanding is essential for unraveling cellular metabolism and pinpointing potential bottlenecks or targets for metabolic engineering.

Protein Structure Studies: In the realm of protein structure and dynamics, this labeled amino acid emerges as an indispensable tool for NMR spectroscopy-based investigations. Incorporating L-Alanine-[1-13C,3,3,3-d3] enhances the sensitivity and resolution of NMR spectra, empowering researchers to delve deeply into protein folding, conformational changes, and interactions with precision. This deeper insight into protein behavior is paramount for drug design and functional analysis, pushing the boundaries of structural biology.

Quantitative Proteomics: Serving as a cornerstone in quantitative proteomics, L-Alanine-[1-13C,3,3,3-d3] acts as an internal standard, facilitating mass spectrometry-based analysis by enabling precise quantification of protein abundance. By juxtaposing the labeled standard with unlabeled peptides in intricate samples, researchers achieve heightened accuracy and reproducibility in protein quantification. This methodology aids in biomarker discovery and unveils proteome dynamics, paving the way for advancements in the proteomics landscape.

Isotopic Labeling in Biosynthesis: Playing a pivotal role in biosynthetic explorations, L-Alanine-[1-13C,3,3,3-d3] stands as a critical tool in investigating the biosynthesis of metabolites and macromolecules. Introducing labeled alanine into microbial or plant systems enables researchers to trace the integration of labeled atoms into diverse biochemical products, unraveling the intricate pathways and mechanisms of biosynthesis. This application yields invaluable insights into natural product formation and synthetic biology, pushing the boundaries of biosynthetic research.