L-Alanine-[13C3,15N,d4]

L-Alanine-[13C3,15N,d4], a stable isotope-labeled amino acid with versatile applications across scientific disciplines, serves as a powerful tool for advancing knowledge. Here are practical applications of L-Alanine-[13C3,15N,d4] presented with high perplexity and burstiness:

Metabolic Flux Analysis: Delving into the intricacies of metabolic flux analysis, researchers leverage L-Alanine-[13C3,15N,d4] to precisely trace the movements of carbon and nitrogen within cellular metabolic pathways. By incorporating this labeled amino acid into cellular systems, scientists quantitatively analyze metabolic pathways, revealing profound insights into cellular metabolism and energy balance. This comprehensive exploration unveils how cells utilize nutrients and adapt to changing environmental cues.

Protein Dynamics Studies: In the realm of protein dynamics research, the integration of L-Alanine-[13C3,15N,d4] offers valuable insights into protein folding, structure, and interactions. By introducing this labeled alanine into proteins, researchers utilize NMR spectroscopy to investigate protein motions and conformational changes. This wealth of information is essential for understanding protein function and designing drugs targeting specific protein interactions, driving advancements in drug discovery.

Environmental Carbon Cycling Research: Acting as a potent tracer in environmental studies, L-Alanine-[13C3,15N,d4] plays a pivotal role in unraveling the dynamics of carbon cycling in ecological systems. By utilizing this isotopically labeled amino acid, researchers track carbon flow through ecosystems, soils, and microbial communities, revealing the role of amino acids in carbon sequestration and the broader carbon cycle in nature. This application sheds light on the intricate balance of carbon dynamics in the environment, guiding conservation efforts.

Stable Isotope Labeling in Mass Spectrometry: Within the realm of mass spectrometry, L-Alanine-[13C3,15N,d4] serves as a critical internal standard for accurately quantifying amino acids in complex biological samples. By leveraging its isotopic labeling, researchers achieve precise measurement of alanine levels, enabling exact quantification even in the presence of structural analogs. This technique proves invaluable in metabolomics and proteomics studies, facilitating accurate measurement and comparison of metabolite concentrations, enriching our understanding of complex biological systems.