L-Aspartic acid-[1-13C,15N]

L-Aspartic acid-[1-13C,15N], a labeled amino acid applied in biochemical and biophysical research, plays a crucial role in various domains. Here are key applications of L-Aspartic acid-[1-13C,15N], each presented with high perplexity and burstiness:

Metabolic Tracing: In the intricate realm of metabolic studies, L-Aspartic acid-[1-13C,15N] emerges as a potent tool for tracing the aspartic acid's journey through complex metabolic pathways. Researchers harness mass spectrometry and NMR spectroscopy to track the incorporation of this labeled amino acid into a multitude of metabolic intermediates, shedding illuminating insight on metabolic fluxes and the dynamic nuances of metabolism across diverse physiological landscapes.

Protein Structure Analysis: Within the captivating domain of NMR spectroscopy, this labeled amino acid assumes a pivotal role in unraveling protein structures. By integrating L-Aspartic acid-[1-13C,15N] into proteins, researchers gain intricate glimpses into the environments and interactions of aspartic acid residues. This dataset proves paramount in deciphering the kinetics of protein folding and elucidating the elaborate dance between proteins and ligands.

Enzyme Mechanism Studies: Spearheading enzyme kinetics exploration, L-Aspartic acid-[1-13C,15N] provides a gateway into the catalytic mechanisms governing aspartate-dependent enzymes. By scrutinizing isotopic shifts in reaction intermediates, researchers delve deep into substrate binding, transition states, and reaction pathways, unveiling invaluable insights for crafting enzyme regulators and deepening our grasp of enzyme functionalities.

Isotope Ratio Analysis: Positioned as the pinnacle in isotope ratio analysis, L-Aspartic acid-[1-13C,15N] empowers the precise measurement of 13C/12C and 15N/14N ratios within biological samples. This technique stands as a cornerstone in exploring nutrient utilization, ecological dynamics, and the intricate tracing of nutrient origins in environmental examinations. The accurate evaluation of isotope ratios propels research in biochemical ecology, fostering a deeper comprehension of nutrient cycling mechanisms and ecological interconnectedness.