L-Aspartic acid-[3-13C]

L-Aspartic acid-[3-13C], a specialized form of L-aspartic acid enriched with carbon-13 at the third position, finds diverse applications in research and analysis. Here are the key applications of L-Aspartic acid-[3-13C]:

Metabolic Flux Analysis: Employing L-Aspartic acid-[3-13C] in metabolic flux analysis enables researchers to meticulously trace and quantify the intricate pathways of metabolic reactions within cells. By introducing this labeled amino acid into cell cultures, scientists can meticulously monitor the carbon flow through complex metabolic networks, unraveling profound insights into cellular metabolism and pinpointing potential targets for metabolic engineering.

NMR Spectroscopy: In the realm of nuclear magnetic resonance (NMR) spectroscopy, L-Aspartic acid-[3-13C] emerges as a valuable tool for probing protein structure and dynamics. The carbon-13 label heightens the resolution and sensitivity of NMR signals, facilitating in-depth examination of the aspartic acid residues within proteins. This enhanced capability leads to a deeper comprehension of protein folding, interactions, and conformational transitions, shedding light on the intricate world of proteins.

Isotopic Labeling Studies: The utilization of L-Aspartic acid-[3-13C] in isotopic labeling studies serves as a powerful method to delve into amino acid biosynthesis and turnover rates in biological organisms. By administering organisms with this labeled compound, researchers can meticulously measure incorporation rates and metabolic transformations, unveiling the intricate biosynthetic pathways and regulatory mechanisms governing amino acids, thus contributing to a comprehensive understanding of cellular processes.

Enzyme Kinetics: Acting as a pivotal substrate in enzyme kinetics studies, L-Aspartic acid-[3-13C] plays a crucial role in assessing the activity and specificity of enzymes interacting with aspartic acid. Through isotopic labeling, researchers can accurately gauge the catalytic mechanisms and reaction rates using advanced techniques like mass spectrometry or NMR. This detailed information is indispensable for unraveling the nuances of enzyme function and laying the groundwork for the development of inhibitors or activators with therapeutic potential.