L-Cysteine-[13C3,d3,15N]

L-Cysteine-[13C3,d3,15N] is an isotopically labeled amino acid used extensively in scientific research. Here are some key applications:

Metabolic Flux Analysis: L-Cysteine-[13C3,d3,15N] is invaluable in tracing metabolic pathways and understanding metabolic fluxes in cellular systems. By incorporating this labeled amino acid into metabolic studies, researchers can identify and quantify the flow of metabolites through various pathways. This information is crucial for optimizing metabolic networks and enhancing our understanding of cellular metabolism.

Stable Isotope-Resolved Metabolomics (SIRM): This isotopically labeled amino acid is employed in SIRM to study the dynamics of metabolites in biological systems. By tracking the incorporation of L-Cysteine-[13C3,d3,15N] into cellular processes, scientists can investigate how different conditions affect metabolic activities. This technique is particularly useful in cancer research, where metabolic reprogramming is a key feature of disease progression.

Protein Structure and Dynamics: L-Cysteine-[13C3,d3,15N] is used in NMR spectroscopy to study the structure and dynamics of proteins. The isotopic labeling enhances the resolution and sensitivity of NMR signals, allowing for detailed analysis of protein folding, interactions, and conformational changes. This application is essential for understanding protein function and for drug discovery efforts.

Nutritional and Environmental Studies: Researchers utilize L-Cysteine-[13C3,d3,15N] in ecological and nutritional studies to track the fate and assimilation of sulfur-containing compounds. By monitoring the incorporation of labeled cysteine into various organisms and ecosystems, scientists can gain insights into nutrient cycling and the impact of environmental changes. This helps in formulating strategies for sustainable agriculture and ecosystem management.