L-Cysteine-[15N]

L-Cysteine-[15N], an isotopically-labeled derivative of the amino acid cysteine, where the nitrogen in the amino group is substituted with the stable nitrogen isotope nitrogen-15, finds diverse applications in various fields. Here are the key applications of L-Cysteine-[15N]:

Metabolic Flux Analysis: Delving into metabolic pathways, L-Cysteine-[15N] serves as a vital tool for metabolic flux analysis. By introducing this labeled amino acid into cellular systems, researchers can meticulously track and quantify nitrogen flux across different metabolic routes. This approach sheds light on nitrogen assimilation and its intricate dispersion within metabolic networks, offering invaluable insights into cellular metabolism, especially in studies centered on amino acid synthesis and breakdown dynamics.

Protein Structure Determination: In the realm of structural biology, the adoption of L-Cysteine-[15N] in NMR spectroscopy revolutionizes the study of protein structures. The strategic isotope labeling elevates the precision and resolution of NMR spectra, enabling in-depth exploration of protein folding dynamics and structural nuances. This analytical prowess is pivotal for unraveling the complexities of protein functionality, molecular interactions, and structural stability, propelling advances in protein science.

Enzyme Mechanism Studies: Within the domain of enzymology, L-Cysteine-[15N] emerges as a prized asset for unraveling the intricacies of enzyme mechanisms and active sites. By monitoring the incorporation and transformation of labeled nitrogen during enzymatic processes, researchers glean critical insights into substrate recognition, catalytic pathways, and intermediate species formation. This detailed understanding enhances the comprehension of enzyme functionality and informs the design of tailored enzyme modulators, be it inhibitors or activators for therapeutic applications.

Nutritional and Health Research: In the realm of nutritional investigations, L-Cysteine-[15N] plays a pivotal role in elucidating protein turnover dynamics and amino acid metabolism in both human and animal systems. By tracking the labeled nitrogen within dietary interventions, scientists can quantify rates of protein synthesis and degradation, unraveling essential information concerning metabolic health, muscle maintenance, and the impact of nutritional supplements. This approach deepens our understanding of the intricate interplay between nutrition and physiological well-being, paving the way for innovative health interventions and dietary strategies.