L-Cysteine-[2,3,3-d3,15N] is a stable isotope-labeled amino acid widely used in scientific research. Here are some key applications of L-Cysteine-[2,3,3-d3,15N]:
Metabolomic Studies: L-Cysteine-[2,3,3-d3,15N] is used in metabolomic experiments to trace and quantify metabolic pathways. By incorporating this labeled amino acid, researchers can track its uptake and conversion within biological systems. This helps in understanding complex metabolic interactions and the role of cysteine in cellular processes.
Protein Structural Analysis: In proteomics, L-Cysteine-[2,3,3-d3,15N] can be used to study protein structure and dynamics. The incorporation of stable isotopes allows for more precise characterization using NMR spectroscopy and mass spectrometry. Researchers gain detailed insights into protein folding, stability, and intermolecular interactions.
Pharmacokinetic Studies: L-Cysteine-[2,3,3-d3,15N] is employed in pharmacokinetic research to study the absorption, distribution, metabolism, and excretion of drugs. By labeling drugs or metabolites with stable isotopes, scientists can accurately track their behavior in vitro and in vivo. This information is crucial for drug development and optimizing therapeutic regimens.
Enzyme Mechanism Investigation: The labeled L-Cysteine-[2,3,3-d3,15N] is used to explore enzyme-catalyzed reactions in biochemistry. Researchers can analyze the catalytic pathways and identify intermediates by tracing the labeled atoms during reaction cycles. This aids in understanding enzyme specificity, mechanism, and potential points for therapeutic intervention.
![L-Cysteine-[2,3,3-d3,15N]](https://resource.bocsci.com/structure/1795787-05-1.gif)
