L-Glutamic acid-[1-13C,15N]

L-Glutamic acid-[1-13C,15N], an isotopically labeled amino acid with diverse scientific and industrial uses, plays a pivotal role in a range of applications. Here are the key applications of L-Glutamic acid-[1-13C,15N].

Metabolic Flux Analysis: Within the realm of metabolic research, L-Glutamic acid-[1-13C,15N] serves as a valuable tool for tracing metabolic pathways and quantifying carbon and nitrogen fluxes within cellular systems. Through the utilization of this labeled compound, scientists can precisely measure the rates of metabolic reactions, gaining insights into the intricate networks of cellular metabolism. This knowledge is indispensable for the development of innovative metabolic engineering strategies and the optimization of diverse biotechnological processes.

NMR Spectroscopy: In the domain of nuclear magnetic resonance (NMR) spectroscopy, L-Glutamic acid-[1-13C,15N] stands out as a key component for exploring protein structure and dynamics. The incorporation of isotopic labels enhances sensitivity and resolution, empowering researchers to map the precise atomic positions within proteins. This technique plays a critical role in unraveling the complex interactions and conformational changes that govern protein folding processes, providing essential insights into the world of protein biochemistry.

Protein Folding Studies: Delving into the intricacies of protein biochemistry, L-Glutamic acid-[1-13C,15N] acts as a fundamental tool for investigating the kinetics and mechanisms underlying protein folding. By monitoring the incorporation and spatial arrangement of labeled amino acids, scientists can meticulously track the journey of proteins as they achieve their unique three-dimensional structures. This research is pivotal for enhancing our understanding of diseases stemming from protein misfolding and shaping the design of potential therapeutic interventions.

Stable Isotope Tracer Experiments: Leveraging the stable isotope tracer capabilities of L-Glutamic acid-[1-13C,15N], researchers embark on groundbreaking explorations in biological and environmental studies. By evaluating nutrient cycling, microbial metabolism, and ecosystem dynamics through the observation of labeled compound metabolization and incorporation into diverse biological materials, valuable insights are gained into the intricate ecological processes and nutrient dynamics across varied ecosystems, pushing the boundaries of scientific understanding.