L-Glutamic acid-[13C5,15N]

L-Glutamic acid-[13C5,15N], a stable isotope-labeled compound, holds diverse applications in bioscience and research. Here are the key applications of L-Glutamic acid-[13C5,15N]:

Metabolic Flux Analysis: By incorporating L-Glutamic acid-[13C5,15N] into metabolic flux analysis, researchers can meticulously track the flow of carbon and nitrogen through metabolic pathways. This tracer enables the detailed quantification of metabolic changes and flux distributions within cellular systems, unveiling the intricate dynamics of cellular metabolism and identifying potential bottlenecks that could impact physiological processes.

Protein Structure Elucidation: In the realm of structural biology, L-Glutamic acid-[13C5,15N] serves as a valuable asset in nuclear magnetic resonance (NMR) spectroscopy for unraveling protein structures. The isotopic labels provide specific signals that aid in mapping out the three-dimensional configurations of proteins and their complexes, offering insights into protein folding kinetics and molecular interactions at the atomic scale. This technique deepens our understanding of the structural complexities of biological macromolecules.

Nutritional Studies: Within the domain of nutritional research, L-Glutamic acid-[13C5,15N] is utilized to explore amino acid metabolism and protein turnover in living organisms. By tracing the labeled glutamic acid, researchers can evaluate rates of protein synthesis and degradation under varying dietary conditions. This data is critical for optimizing nutritional interventions, improving health outcomes, and shedding light on the physiological mechanisms governing protein metabolism in living systems.

Biomedical Research: In the landscape of biomedical investigations, L-Glutamic acid-[13C5,15N] serves as a powerful tracer for delving into cellular and molecular processes such as neurotransmitter cycling and glutamine-glutamate metabolism in the brain. Understanding these intricate metabolic pathways is essential for unraveling the underlying mechanisms of neurological disorders and designing targeted therapeutic strategies. This isotope-labeled compound forms the foundation of cutting-edge research in neuroscience and medicine, unlocking profound discoveries in the field of biomedicine.