L-Glutamic acid-[13C5,d5,15N] is a stable isotope-labeled amino acid used in various scientific and research applications. Here are some key applications of L-Glutamic acid-[13C5,d5,15N]:
Metabolic Flux Analysis: L-Glutamic acid-[13C5,d5,15N] is utilized in metabolic flux analysis to trace carbon and nitrogen pathways in cellular metabolism. By incorporating this isotopically labeled compound into cell cultures, researchers can follow its incorporation and breakdown, providing insights into metabolic networks. This application helps in understanding cellular physiology and optimizing metabolic engineering processes.
Protein Structure and Dynamics: In structural biology, L-Glutamic acid-[13C5,d5,15N] is used to investigate protein structure and dynamics through NMR spectroscopy. The labeled glutamic acid allows for the precise identification of amino acid residues within a protein, revealing critical details about its conformation and interactions. These studies are essential for drug design and functional analyses of proteins.
Environmental and Ecological Studies: L-Glutamic acid-[13C5,d5,15N] is employed in ecological research to study nitrogen cycling and nutrient dynamics in ecosystems. By tracking the isotope-labeled nitrogen, scientists can analyze the uptake and transformation of nitrogen compounds by plants and microorganisms. This information is crucial for understanding nutrient flows and ecosystem health.
Biomedical Research: In biomedical applications, L-Glutamic acid-[13C5,d5,15N] is used to study amino acid metabolism and its role in various diseases. By incorporating this labeled compound into cellular or animal models, researchers can analyze alterations in glutamate metabolism associated with conditions such as neurological disorders and cancer. This knowledge can inform the development of therapeutic strategies and diagnostic tools.
![L-Glutamic acid-[13C5,d5,15N]](/isotope.v1/images/structure-default.jpg)
