Boc-Glu(OBzl)-OH-[15N]

Boc-Glu(OBzl)-OH-[15N], a commonly used isotopically labeled derivative in biochemical and pharmaceutical research, offers a multitude of applications across various domains. Here are the key applications:

Protein Structure Elucidation: Leveraging Boc-Glu(OBzl)-OH-[15N] in nuclear magnetic resonance (NMR) spectroscopy unveils a powerful tool for unraveling intricate protein structures. The integration of the nitrogen-15 isotope enables a comprehensive examination of nitrogen-containing functional groups, pivotal for deciphering protein folding dynamics and intricate molecular interactions.

Metabolic Flux Analysis: Utilizing this isotopically labeled compound in metabolic investigations propels the study of nitrogen flow within metabolic pathways to new heights. By monitoring the assimilation of nitrogen-15 into diverse metabolic intermediates, researchers embark on a journey to illuminate nitrogen assimilation and utilization within biological systems, shedding light on cellular metabolism and the intricate cycles of nutrients.

Drug Development and Pharmacokinetics: The application of Boc-Glu(OBzl)-OH-[15N] emerges as a cornerstone in drug development, offering unparalleled insights into drug metabolism and distribution dynamics. Isotope-labeled compounds pave the way for in-depth exploration of the pharmacokinetics of novel drug candidates, facilitating a comprehensive analysis of absorption, distribution, metabolism, and excretion (ADME) properties. This exploration serves as a crucial guide in optimizing pharmaceutical compounds and ensuring their safety profile.

Peptide Synthesis: Within the realm of peptide chemistry, Boc-Glu(OBzl)-OH-[15N] assumes a pivotal role as a fundamental building block for crafting nitrogen-15 labeled peptides. These specialized peptides find application across structural studies and assays exploring enzyme-substrate interactions, elevating the precision and sensitivity of analytical techniques like mass spectrometry and NMR spectroscopy to unprecedented levels.