L-Glutamine-[1-13C,alpha-15N]

L-Glutamine-[1-13C,alpha-15N], an isotopically labeled amino acid, finds applications in diverse biochemical and medical research realms. Here are the key applications of L-Glutamine-[1-13C,alpha-15N]:

Metabolic Flux Analysis: Engaging L-Glutamine-[1-13C,alpha-15N] in metabolic investigations enables researchers to map and quantify intricate metabolic pathways. By infusing this labeled amino acid into cellular metabolism, scientists can meticulously monitor its conversions and unravel the intricate web of metabolites. This analytical approach sheds light on the dynamic nature of cellular metabolism and pinpoints critical metabolic bottlenecks.

NMR Spectroscopy: A valuable asset in nuclear magnetic resonance (NMR) spectroscopy, this isotopically labeled L-Glutamine is a staple for exploring protein structure and dynamics. The 13C and 15N isotopic labels contribute to heightened spectral precision and sensitivity, facilitating in-depth examinations of protein folding, interactions, and functional states. This detailed insight is pivotal for deciphering the molecular mechanisms underpinning protein functions.

Cancer Research: At the forefront of oncology research, L-Glutamine-[1-13C,alpha-15N] serves as a cornerstone for investigating tumor metabolism and the impact of glutamine on cancer cell proliferation. Through meticulous tracking of labeled glutamine uptake and utilization, researchers delve into the metabolic dependencies of cancer cells, identifying potential targets for therapeutic intervention. This innovative approach fuels the development of metabolic strategies for combating cancer.

Nutritional Studies: By leveraging L-Glutamine-[1-13C,alpha-15N], scientists embark on enlightening journeys into the metabolism of glutamine across diverse nutritional contexts. Through meticulous examinations of how the body processes this labeled amino acid, researchers gain profound insights into its contributions to muscle protein synthesis, immune function, and overall well-being. This research underpins the formulation of nutritional supplements and dietary guidelines, shaping the landscape of holistic health practices.