L-Glutamine-[15N2,d10] is a stable isotope-labeled compound used extensively in bioscience research to investigate metabolic pathways and protein synthesis. Here are some key applications of L-Glutamine-[15N2,d10]:
Metabolic Flux Analysis: In metabolic research, L-Glutamine-[15N2,d10] is used as a tracer to study nitrogen flow through metabolic pathways. By tracking the incorporation of this labeled glutamine into nitrogen-containing compounds, researchers can analyze metabolic fluxes and understand the dynamics of nitrogen metabolism. This information is crucial for optimizing cellular processes in biotechnology and understanding disease metabolism.
Protein Synthesis Studies: Labeled isotopes like L-Glutamine-[15N2,d10] are invaluable in protein synthesis research, allowing scientists to trace the incorporation of nitrogen into newly synthesized proteins. Researchers can quantify and analyze protein turnover rates and gain insights into cellular protein dynamics under various conditions. This has significant implications for understanding diseases related to protein synthesis dysregulation.
Nutritional Research: L-Glutamine-[15N2,d10] can be employed in nutritional studies to explore the role of glutamine in human health and disease. By monitoring the metabolism of this isotope in the body, researchers can assess how dietary glutamine is utilized and its effect on muscle health, immune function, and overall metabolism. This can lead to better nutritional guidelines and therapeutic strategies.
Cell Culture Studies: In vitro, L-Glutamine-[15N2,d10] is used to study the metabolic requirements of cultured cells, including cancer cells, for glutamine. By supplying this labeled compound in the culture medium, scientists can gather data on glutamine uptake, utilization, and its role in cell proliferation and survival. This research may identify metabolic vulnerabilities that can be targeted in cancer treatment.
![L-Glutamine-[15N2,d10]](https://resource.bocsci.com/structure/l-glutamine-%5B15n2%2Cd10%5D.gif)
