L-Histidine-[13C6,α-amine-15N]

L-Histidine-[13C6,α-amine-15N], a stable isotope-labeled amino acid, finds versatile use in various scientific applications. Here are key applications of L-Histidine-[13C6,α-amine-15N] presented with high perplexity and burstiness:

Metabolic Flux Analysis: Widely deployed in metabolic flux analysis, this isotopically labeled amino acid serves as a powerful tool for tracing biochemical pathways within cells. By integrating L-Histidine-[13C6,α-amine-15N] into cellular metabolism, researchers gain the ability to track the distribution and metamorphosis of carbon and nitrogen atoms in intricate metabolic networks. Such utilization offers profound insights into cellular metabolism, empowering scientists to fine-tune metabolic pathways for advancements in biotechnology and drug development.

Protein Structure and Dynamics: In the realm of nuclear magnetic resonance (NMR) spectroscopy, L-Histidine-[13C6,α-amine-15N] plays a pivotal role in studying protein structure and dynamics at a granular level. The distinctive stable isotopic labeling facilitates in-depth examination of protein folding, interactions, and conformational transitions, shedding light on the nuances of protein function. This application holds critical significance in unraveling protein structures and designing therapeutic agents tailored to specific protein architectures.

Biomolecular Research: Within the domain of biomolecular research, L-Histidine-[13C6,α-amine-15N] emerges as a key player in studying the intricate web of protein-protein and protein-ligand interactions. The labeled amino acid acts as a beacon, enabling precise identification and quantification of binding sites, thereby furnishing pivotal data for the discovery and development of novel drugs. Insights gleaned from such investigations provide a springboard for crafting more efficacious drugs with targeted modes of action.

Nutritional Studies: Delving into the realms of nutritional science, L-Histidine-[13C6,α-amine-15N] serves as a cornerstone in exploring amino acid utilization and turnover dynamics within the human body. By meticulously tracing the metabolic destiny of this labeled amino acid, researchers can evaluate protein metabolism and assess dietary protein requisites. This application serves as a compass for deciphering the essential amino acid requirements, particularly in clinical settings where dietary interventions play a pivotal role in optimizing health outcomes.