L-Histidine-[13C6,D5,15N3] hydrochloride monohydrate

L-Histidine-[13C6,D5,15N3] hydrochloride monohydrate is an isotopically labeled amino acid used extensively in biochemical research. Here are some key applications of L-Histidine-[13C6,D5,15N3] hydrochloride monohydrate:

Metabolic Flux Analysis: L-Histidine-[13C6,D5,15N3] hydrochloride monohydrate is integral in studying metabolic fluxes within cellular systems. By incorporating this labeled compound into experiments, researchers can trace the incorporation of histidine into metabolic pathways. This helps in understanding how cells distribute and utilize nutrients, providing insights into various metabolic disorders.

Protein Structure and Function Studies: This isotopically labeled form of histidine is used in NMR spectroscopy to study protein structures and dynamics. By substituting natural histidine residues with L-Histidine-[13C6,D5,15N3], scientists can obtain detailed information about the environment and interactions of histidine residues within proteins. This is essential for uncovering protein folding mechanisms and functional site analyses.

Enzyme Mechanism Investigation: Researchers use L-Histidine-[13C6,D5,15N3] hydrochloride monohydrate in studies aimed at understanding enzyme mechanisms. By labeling specific histidine residues in enzyme active sites, it becomes possible to track their roles during catalysis. This information is crucial for designing enzyme inhibitors and understanding enzyme catalysis in greater detail.

Stable Isotope-Labeling Studies: In mass spectrometry-based proteomics, L-Histidine-[13C6,D5,15N3] is employed for isotope labeling to quantify protein abundances. This quantitative approach allows researchers to compare protein expression levels across different conditions or treatments. Such studies are pivotal in biomarker discovery and understanding protein regulation in health and disease.