L-Histidine-[amine-15N]

L-Histidine-[amine-15N], a stable isotope-labeled amino acid, is highly utilized in diverse scientific applications. Here are key applications of L-Histidine-[amine-15N] presented with elevated perplexity and burstiness:

Metabolic Tracing: A pivotal tool in studying metabolic pathways, L-Histidine-[amine-15N] enables the tracing of nitrogen integration into a myriad of biomolecules. Researchers leverage this compound to track the destiny of nitrogen within histidine metabolism or related pathways in living organisms. This method offers profound insights into the variances in amino acid metabolism under various physiological or pathological conditions, shedding light on the intricacies of biochemical processes.

Protein Research: Within the realm of protein studies, L-Histidine-[amine-15N] functions as a crucial isotope label for NMR spectroscopy. By incorporating this labeled amino acid into proteins, scientists embark on detailed structural and dynamic analyses. This intricate process aids in unraveling the mysteries of protein folding, interactions, and function at an atomic level, essential for the realms of drug design and biochemical explorations.

Nutritional Studies: Applied in nutritional investigations, L-Histidine-[amine-15N] plays a vital role in evaluating dietary protein utilization and amino acid metabolism in both humans and animals. By scrutinizing the integration of L-Histidine-[amine-15N] into bodily tissues, researchers delineate protein turnover rates and assess nutritional statuses. These comprehensive studies underpin the formulation of dietary guidelines and the implementation of therapeutic nutritional strategies, enriching our understanding of the intricate interplay between nutrition and health.

Microbial Ecology: Within the domain of microbial ecology, L-Histidine-[amine-15N] emerges as a prominent tracer for studying nitrogen assimilation and turnover within microbial communities. By monitoring the labeled nitrogen within microbial biomass, scientists delve into the complexities of nutrient cycling and microbial activity across diverse environments. This invaluable data not only enhances our comprehension of ecosystem functions but also informs environmental management practices, shaping sustainable approaches towards ecosystem preservation.