L-Histidine-[ring-2,4-d2,alpha,beta,beta-d3] hydrochloride monohydrate

L-Histidine-[ring-2,4-d2,alpha,beta,beta-d3] hydrochloride monohydrate is a labeled amino acid used in various scientific research applications. Here are some key applications of this compound:

Metabolic Tracing: This isotopically labeled form of L-Histidine is widely used in metabolic studies to trace and analyze metabolic pathways. By incorporating this labeled compound into experimental systems, researchers can track its conversion and distribution across different biochemical reactions. This provides insights into amino acid metabolism, protein synthesis, and turnover rates in various organisms.

Protein Structure Analysis: L-Histidine-[ring-2,4-d2,alpha,beta,beta-d3] hydrochloride monohydrate is valuable in NMR spectroscopy studies aimed at elucidating protein structures. The deuterium labeling allows for clearer spectral resolution and aids in identifying specific interactions within proteins. Researchers use this data to understand protein folding, stability, and function, which are crucial for drug development and understanding diseases at the molecular level.

Enzyme Kinetics: This labeled compound can be employed in studies investigating enzyme kinetics and mechanisms. By substituting it into enzyme substrates, scientists can monitor reaction rates and intermediate formations with enhanced precision. Such studies are essential for characterizing enzyme activity, developing inhibitors, and improving industrial enzymatic processes.

Cellular and Molecular Biology: L-Histidine-[ring-2,4-d2,alpha,beta,beta-d3] hydrochloride monohydrate is used in cell culture and molecular biology experiments. Researchers incorporate it into cultures to study its effects on cell growth, protein synthesis, and metabolic activity in labeled versus non-labeled conditions. This application helps in understanding cellular responses to environmental changes and in designing experiments for metabolic engineering.